SET8 / PR-SET7 antibody (pAb)

for SET8 / PR-SET7 antibody (pAb)Background

SET8 (SET-domain containing protein 8, PR-SET7, SETD8, KMT5A) is a SET domain-containing histone methyltransferase that specifically monomethylates histone H4 Lys20. H4 Lys20 monomethylation is enriched during mitosis and is associated with transcriptional repression. SET8 mainly functions in euchromatic regions, playing a role in gene silencing. SET8 knockout is homozyhgous lethal in mice and its function is required for cell cycle progression and cell proliferation. SET8 is required for proper chromosome condensation during interphase and chromosome segregation during mitosis. SET8 is also involved in DNA damage repair, as cells lacking SET8 function accumulate massive amounts of DNA damage. SET8 has been found to methylate p53 at Lys382, indicating a potential mechanism for SET8s role in the DNA damage repair pathway.

for SET8 / PR-SET7 antibody (pAb)Immunogen

This SET8 / PR-SET7 antibody was raised against a peptide corresponding to amino acids within the N-terminal half of human SETD8.

for SET8 / PR-SET7 antibody (pAb)Application Notes

This SET8 / PR-SET7 antibody has been validated for use in Western blotting (1:500 – 1:1,000).

for SET8 / PR-SET7 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for SET8 / PR-SET7 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SET8 / PR-SET7 antibody.

for SET8 / PR-SET7 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SET8 / PR-SET7 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.