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ERR-α antibody (pAb)

Aliases: ERR1, ERRa, ESRL1, NR3B1, ERRalpha
商品コード: 61047 Format: 100 µl ¥67,000 Buy Now
商品コード: 61048 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:50 kDa
Reactivity:Human

Applications

Western Blot Validated

for ERR-a antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for ERR-a antibody (pAb)Immunogen

This ERR-alpha antibody was raised against a peptide within the C-terminal region of human ERR alpha.

for ERR-a antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ERR-a antibody (pAb)References

 
ERR-α antibody (pAb) tested by Western blot.

Western blot of ERR-α antibody.
Nuclear extract of MDA-MB-231 cells (20 µg per lane) probed with ERR alpha antibody at a dilution of 1:500.

for ERR-a antibody (pAb)Background

ERR-α (Estrogen-related receptor alpha, NR3B1) is an orphan nuclear receptor similar to the Estrogen Receptor. It is a sequence-specific DNA binding transcriptional activator that is constitutively active in the absence of ligand binding. No true physiological ligands have been found, but estrogen-like compounds from plants have been shown to activate ERR-α. ERR-α and ER-α have been found to regulate an overlapping set of genes, and ERR-α appears to modulate the activity of ER-α in some tissue types. ERR-α regulates genes involved in mitochondrial biogenesis, gluconeogenesis, oxidative phosphorylation, and fatty acid metabolism. ERR-α knockout mice display impaired fat metabolism and absorption.

for ERR-a antibody (pAb)Positive Control

Active Motif's MDA-MB-231 nuclear extract (Catalog No. 36080) can be used as a positive control for ERR-α antibody.

for ERR-a antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ERR-a antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ERR-α antibody (pAb)
 

Data Thumbnails

ERR-α antibody (pAb) tested by Western blot.

Western blot of ERR-α pAb.
(Click image to enlarge and see details.)