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RNA pol II CTD phospho Ser2 antibody (mAb)

Clone: 3E10
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61083 Format: 100 µg ¥69,000 Buy Now
商品コード: 61084 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:240 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Application Notes


Validated Applications:
ChIP: 20 µg per ChIP
ChIP-Seq: 20 µg each

Published Applications:
ChIP
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Immunogen

This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91115.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)References

 
RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP.

RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 10 µg of chromatin from human myeloma LP1 cells and 20 µg RNA pol II CTD phospho Ser2 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq.

RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2.3 million HL-60 cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify RNA pol II phospho Ser2 binding. Data is compared to ChIP-Seq data using a phospho Ser5 antibody (61085). ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser2 antibody detects polymerase more toward the 3´ end of the genes and the phospho Ser5 antibody detects Pol II more at the 5´ end of the genes.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Ser2 antibody.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-1 (Cat. No. 71003) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-1 (Cat. No. 71015) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA pol II CTD phospho Ser2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RNA pol II CTD phospho Ser2 antibody (mAb).

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA pol II CTD phospho Ser2 antibody (mAb)
 

Data Thumbnails

RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP.

ChIP of RNA pol II CTD phospho Ser2 mAb.
(Click image to enlarge and see details.)

RNA pol II CTD phospho Ser2 antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of RNA pol II CTD phospho Ser2 mAb.
(Click image to enlarge and see details.)