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STAT5A antibody (pAb)

Aliases: MGF, STAT5
商品コード: 61133 Format: 100 µg ¥67,000 Buy Now
商品コード: 61134 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:92 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for STAT5A antibody (pAb)Application Notes


Validated Applications:
IP: 10 µg per IP
WB: 0.5 - 2 µg/ml dilution

for STAT5A antibody (pAb)Immunogen

This STAT5A antibody was raised against a peptide in the C-terminal region of human STAT5A.

for STAT5A antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT5A antibody (pAb)References

 
STAT5A antibody (pAb) tested by immunoprecipitation.

STAT5A antibody (pAb) tested by Immunoprecipitation.
10 µg of STAT5A antibody was used to immunoprecipitate STAT5A from 400 µg of K562 cytosolic cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT5A antibody at a dilution of 1 µg/ml.

STAT5A antibody (pAb) tested by Western blot.

STAT5A antibody (pAb) tested by Western blot.
STAT5A antibody detection by Western blot. The analysis was performed using K-562 cytosolic-cell extract (25 µg) and STAT5A antibody (pAb) at a 1 µg/ml dilution.

for STAT5A antibody (pAb)Background

STAT5A and STAT5B are encoded by separate genes but are 90% identical. They mediate gene expression in response to IL-2, IL-3, IL-7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. The STAT5A/B signaling pathway is involved in the transition of organ-confined prostate cancer to hormone-refractory disease. This implies that the active JAK2-STAT5A/B signaling pathway potentially provides the means for pharmacological intervention of clinical prostate cancer progression.

for STAT5A antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT5A antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT5A antibody (pAb)
 

Data Thumbnails

STAT5A antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of STAT5A pAb.
(Click image to enlarge and see details.)

STAT5A antibody (pAb) tested by Western blot.

Western blot of STAT5A pAb.
(Click image to enlarge and see details.)