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Histone H4R3me2s (symmetric) antibody (pAb)

商品コード: 61187 Format: 100 µl ¥67,000 Buy Now
商品コード: 61188 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:10 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Dot Blot Validated

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Immunogen

This Histone H4 dimethyl Arg3 (H3R3me2s) antibody was raised against a peptide containing dimethyl Arg3 (symmetric) of human Histone H4.

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)References

 
Histone H4R3me2s (symmetric) antibody (pAb) tested by Western blot.

Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot.
Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 µg). Lane 2: Nuclear extract from HeLa cells (20 µg).

Histone H4R3me2s (symmetric) antibody (pAb) tested by dot blot analysis.

Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.

Symmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional repression. Histone H4 dimethyl Arg3 symmetric methylation (H4R2me2s) is catalyzed by PRMT5 and PRMT7.

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H4 dimethyl Arg3, symmetric antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4R3me2s (symmetric) antibody (pAb).

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 dimethyl Arg3 symmetric antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4R3me2s (symmetric) antibody (pAb)
 

Data Thumbnails

Histone H4R3me2s (symmetric) antibody (pAb) tested by Western blot.

Western blot of Histone H4R3me2s (symmetric) pAb.
(Click image to enlarge and see details.)

Histone H4R3me2s (symmetric) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4R3me2s (symmetric) pAb.
(Click image to enlarge and see details.)