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KLF6 antibody (pAb)

Aliases: GBF, ZF9, BCD1, CBA1, CPBP, PAC1, ST12, COPEB
商品コード: 61243 Format: 100 µg ¥67,000 Buy Now
商品コード: 61244 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:40 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for KLF6 antibody (pAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for KLF6 antibody (pAb)Immunogen

This antibody was raised against a peptide within the internal region of human KLF6.

for KLF6 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for KLF6 antibody (pAb)References

 
KLF6 antibody (pAb) tested by Western blot.

KLF6 pAb tested by Western blot.
3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 µg per lane was probed with KLF6 pAb at a dilution of 2 µg/ml .
    Lane 1: Day 0.
    Lane 2: Day 10 - fully differentiated adipocytes.

for KLF6 antibody (pAb)Background

KLF6 (Kruppel-like factor 6) is member of the Kruppel-like family of transcription factors and is a zinc finger protein that acts as a transcriptional activator and functions as a tumor suppressor. Could play a role in B-cell growth and development. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.

for KLF6 antibody (pAb)Positive Control

Active Motif's 3T3-L1 nuclear extract (Catalog No. 36071) can be used as a positive control for KLF6 antibody.

for KLF6 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for KLF6 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

KLF6 antibody (pAb)
 

Data Thumbnails

KLF6 antibody (pAb) tested by Western blot.

Western blot of KLF6 pAb.
(Click image to enlarge and see details.)