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PDX1 antibody (pAb)

Aliases: Pancreatic And Duodenal Homeobox 1, Insulin Promoter Factor 1, Homeodomain Transcription Factor, Somatostatin-Transactivating Factor 1
商品コード: 61289 Format: 100 µl ¥67,000 Buy Now
商品コード: 61290 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:42 kDa
Reactivity:Human

Applications

Western Blot Validated

for PDX1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for PDX1 antibody (pAb)Immunogen

This PDX1 antibody was raised against a peptide within the N-terminal region of human PDX1.

for PDX1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PDX1 antibody (pAb)References

 
PDX1 antibody (pAb) tested by Western blot.

PDX1 pAb tested by Western blot.
PDX1 detection by Western blot. The analysis was performed using 30 µg Caco-2 nuclear cell extract and PDX1 at a 1:500 dilution.

for PDX1 antibody (pAb)Background

PDX1 (pancreatic and duodenal homeobox 1, GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1) activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

for PDX1 antibody (pAb)Positive Control

Active Motif's Caco-2 nuclear extract (Catalog No. 36035) can be used as a positive control for PDX1 antibody.

for PDX1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PDX1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PDX1 antibody (pAb)
 

Data Thumbnails

PDX1 antibody (pAb) tested by Western blot.

Western blot of PDX1 pAb.
(Click image to enlarge and see details.)