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Histone H4H18ph antibody (pAb)

商品コード: 61413 Format: 100 µg ¥67,000 Buy Now
商品コード: 61414 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

for Histone H4H18ph antibody (pAb)Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

for Histone H4H18ph antibody (pAb)Immunogen

This antibody was raised against a synthetic peptide containing a 3-phosphohistidine analog at Histidine 18 of human Histone H4.

for Histone H4H18ph antibody (pAb)Buffer

Purified IgG containing 30% glycerol and 0.035% sodium azide.
This serum was depleted over unmodified peptide before protein A purification.

for Histone H4H18ph antibody (pAb)References

 
Histone H4H18ph antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4 phospho Histidine 18 antibody (pAb).
Dot blot analysis was used to confirm the specificity of H4H18ph antibody for phosphorylation on Histidine 18 of histone H4. Peptides corresponding to the immunogen and an unmodified sequence were spotted onto PVDF and probed with H4H18ph at a 1:1,000 dilution. The amount of pepetide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified peptide.

Lane 2: H4H18ph peptide.

for Histone H4H18ph antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Phosphorylation of histidines in Histone H4 has been known to occur in liver cells and may be associated with proliferation of hepatocytes during regeneration of the liver following damage.

for Histone H4H18ph antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4H18ph antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4H18ph antibody (pAb)
 

Data Thumbnails

Histone H4H18ph antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4H18ph pAb.
(Click image to enlarge and see details.)