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RBPJ antibody (mAb)

Clone: 1F1
Aliases: SUH, csl, AOS3, CBF1, KBF2, RBP-J, RBPJK, IGKJRB, RBPSUH, IGKJRB1
商品コード: 61505 Format: 100 µg ¥67,000 Buy Now
商品コード: 61506 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:68 kDa
Reactivity:Human

Applications

ChIP Validated Immunofluorescence Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

for RBPJ antibody (mAb) (Clone 1F1)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ChIP
EMSA
See references for more information. Individual optimization may be required.

for RBPJ antibody (mAb) (Clone 1F1)Immunogen

This antibody was raised against a full-length recombinant protein corresponding to human RBPJ.

for RBPJ antibody (mAb) (Clone 1F1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RBPJ antibody (mAb) (Clone 1F1)References

 
RBPJ antibody (mAb) tested by ChIP.

RBPJ antibody (mAb) tested by ChIP.
Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.

RBPJ antibody (mAb) tested by immunofluorescence.

RBPJ antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 µg/ml dilution.

for RBPJ antibody (mAb) (Clone 1F1)Background

RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RBPJ antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RBPJ antibody (mAb).

for RBPJ antibody (mAb) (Clone 1F1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RBPJ antibody (mAb) (Clone 1F1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RBPJ antibody (mAb)
 

Data Thumbnails

RBPJ antibody (mAb) tested by ChIP.

ChIP of RBPJ mAb.
(Click image to enlarge and see details.)

RBPJ antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of RBPJ mAb.
(Click image to enlarge and see details.)