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AbFlex® Lamin A/C antibody (rAb)

商品コード: 91189 Format: 100 µg ¥62,000 Buy Now
商品コード: 91190 Format: 10 µg ¥17,000 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Host:Mouse
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated

for AbFlex Lamin A/C antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml
IF: 2 µg/ml

for AbFlex Lamin A/C antibody (rAb)Immunogen

This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

for AbFlex Lamin A/C antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39287.

 
AbFlex<sup>®</sup> Lamin A/C antibody (rAb) tested by Western blot.

AbFlex® Lamin A/C antibody (rAb) tested by Western blot. 10 µg of HeLa cell extract was probed with 1 µg/ml AbFlex Lamin A/C antibody. MW: 80 kDa

AbFlex<sup>®</sup> Lamin A/C antibody (rAb) tested by Western blot.

AbFlex® Lamin A/C antibody (rAb) tested by Immunofluorescence
HeLa cells were stained with 2 µg/ml Lamin A/C antibody (upper panel) or DAPI (lower panel).

for AbFlex Lamin A/C antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® Lamin A/C antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AbFlex® Lamin A/C antibody (rAb).

for AbFlex Lamin A/C antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Lamin A/C antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Lamin A/C antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Lamin A/C antibody (rAb) tested by Western blot.

Western blot of AbFlex® Lamin A/C antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Lamin A/C antibody (rAb) tested by Western blot.

Western blot of AbFlex® Lamin A/C antibody (rAb).
(Click image to enlarge and see details.)