Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

FACE™ AKT

in-cell Western analysis for phospho AKT (S473)

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE AKT Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total AKT. The phospho-AKT antibody recognizes AKT1 only when phosphorylated at Ser473, and AKT2 and AKT3 only when phosphorylated at equivalent sites; the total-AKT antibody recognizes AKT1, AKT2 and AKT3 regardless of the phosphorylation state. Click the AKT Info tab below for data and more information.

 
Name Format Cat No. 価格 (税抜)  
FACE™ AKT 1 x 96 rxns 48120 ¥97,000 Buy Now
5 x 96 rxns 48620 ¥389,000 Buy Now
FACE™ AKT Chemi 1 x 96 rxns 48220 ¥106,000 Buy Now
5 x 96 rxns 48720 ¥427,000 Buy Now
 
Measurement of phosphorylated AKT and total AKT using the FACE AKT Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total AKT.

NIH/3T3 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 50 ng/ml PDGF for 5 minutes and fixed. Total and phospho-AKT were each assayed in triplicate using the phospho and total AKT antibodies from the FACE AKT Kit.

Antibody Specificities

The phospho-AKT antibody is specific for phosphorylated AKT and was raised against a phospho-Ser473 peptide that corresponds to the sequence surrounding Ser473 of mouse AKT. It recognizes AKT1 only when phosphorylated at Ser473, and AKT2 and AKT3 only when phosphorylated at equivalent sites. The total-AKT antibody recognizes AKT1, AKT2 and AKT3 regardless of the phosphorylation state.

AKT Overview

AKT, also known as protein kinase B (PKB) or Related to A and C kinases (RAC), is the cellular homolog of the viral oncogene v-AKT. It was originally cloned based on its homology to protein kinases A and C. AKT is activated in response to insulin, and various growth and survival factors. It is clear that AKT enzyme activity is both necessary and essential for cell survival, and AKT has been shown to be able to induce progression of the cell cycle as well as suppress apoptosis. The key role of AKT in the regulation of cell survival makes it a promising target for anti-oncogenic therapeutics.

FACE™ の使用法

96ウェルプレートで細胞を培養し, 目的の経路を誘導する刺激を与えます。刺激を与えた後, 細胞を固定することでリン酸化を含むタンパク質修飾が保持されます。ブロッキング反応の後, それぞれのウェルに目的タンパク質に特異的な一次抗体, 続いてHRP標識二次抗体を加えます。その

 
FACE flow chart
 
図1: FACE の操作法

Contents & Storage

Two (or ten) 96-well plates for culturing cells, 96 (or 5 x 96) rxns each of two primary antibodies (1 phospho-specific, 1 specific for native protein), HRP-conjugated secondary antibody, Quenching Solution, 1X Antibody Blocking Buffer, 1X Antibody Dilution Buffer, 10X PBS, 10% Triton X-100, 1% SDS Solution, Developing and Stop Solutions, and Crystal Violet Cell Quantification Solution. Storage conditions vary from room temperature to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.