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Recombinant MST1 protein

Aliases: Mammalian STE20-Like Protein Kinase 1, STK4, KRS2, YSK3, TIIAC
商品コード: 31355 Format: 10 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant MST1 proteinContents

10 µg recombinant Mst1 expressed in Sf9 cells and supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant MST1 proteinBackground

MST1 (Mammalian STE20-Like Protein Kinase 1) is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates Ser-14 of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on Ser-212 and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on Ser-650 and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

for Recombinant MST1 proteinApplication Notes

Recombinant MST1 is suitable for use in kinase assays. A good starting point is 5 to 25 ng of enzyme per assay.

for Recombinant MST1 proteinProtein Details

Amino acids 2-488 of MST1 (accession number NP_006273.1) were expressed with an N-terminal GST-Tag, ( 82 kDa) in Sf9 cells using a baculovirus expression system.

 
MST1 protein Coomassie gel

MST1 protein gel.
MST1 (6 μg) run on an SDS-PAGE gel and stained with Coomassie blue.

MST1 activity assay

MST1 activity assay.
Recombinant MST1 activity measured using a fluorescent kinase assay.

for Recombinant MST1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant MST1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant MST1 protein
 

Data Thumbnails

MST1 protein Coomassie gel

MST1 protein Coomassie gel.
(Click image to enlarge and see details).

MST1 activity assay

MST1 activity assay.
(Click image to enlarge and see details).