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Histone H4 pan-acetyl antibody (pAb)
| Catalog No: 39243 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39244 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:10,000 | 5 - 10 µl per ChIP | 1:500 - 1:2,000 |
for Histone H4 pan-acetyl antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.
for Histone H4 pan-acetyl antibody (pAb)Immunogen
This antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).
for Histone H4 pan-acetyl antibody (pAb)Application Notes
This Histone H4 pan-acetyl antibody (H4Ac) has been validated for use in Western blotting (1:1,000 - 1:10,000 dilution), chromatin IP (5 - 10 µl per ChIP) and immunofluorescence (1:500 - 1:2,000 dilution). The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts.
for Histone H4 pan-acetyl antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H4 pan-acetyl antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 pan-acetyl antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 pan-acetyl antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4 pan-acetyl antibody (pAb).
Histone H4 pan-acetyl antibody (pAb) tested by Western blot.
HeLa acid extract (20 µg /lane) probed with Histone H4 pan-acetyl polyclonal antibody (1:10,000 dilution).
Lane 1: Cells treated with sodium butyrate.
Lane 2: No treatment.
Histone H4 pan-acetyl antibody (pAb) tested by immunofluorescence.
Staining of HeLa cells with Histone H4 pan-acetyl pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H4 pan-acetyl antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl pAb for acetyl histone H4. Decreasing amounts of acetylated peptides corresponding to the immunogen, a related sequence, as well as unacetylated histone H4 peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution.
Lane 1: AGG[acK]GG[acK]GMG[acK]VGA[acK]RHSC
Lane 2: AGG[acK]GG[acK]GG[acK]GG[acK]GG[acK]GGC
Lane 3: SGRGKGGKGLC
Lane 4: GKGGAKRHRKC
Histone H4 pan-acetyl antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 2 µl of Histone H4 pan-acetyl pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H4 pan-acetyl antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 pan-acetyl antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
