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Histone H4ac (pan-acetyl) antibody (pAb)

商品コード: 39243 Format: 100 µl ¥67,000 Buy Now
商品コード: 39244 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H4 pan-acetyl antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:1,000 - 1:10,000 dilution

Published Applications:
ChIP
WB
ICC
IF

for Histone H4 pan-acetyl antibody (pAb)Immunogen

This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).

for Histone H4 pan-acetyl antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39925) of this antibody that was purified by Protein A Chromatography is also available.

for Histone H4 pan-acetyl antibody (pAb)References

 
Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP.

Histone H4 pan-acetyl antibody (pAb) tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 µl of Histone H4 pan-acetyl pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence.

Histone H4 pan-acetyl antibody (pAb) tested by immunofluorescence.
Staining of HeLa cells with Histone H4 pan-acetyl pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot.

Histone H4 pan-acetyl antibody (pAb) tested by Western blot.
HeLa acid extract (20 µg /lane) probed with Histone H4 pan-acetyl polyclonal antibody (1:10,000 dilution).
    Lane 1: Cells treated with sodium butyrate.
    Lane 2: No treatment.

Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Histone H4 pan-acetyl antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl pAb for acetyl histone H4. Decreasing amounts of acetylated peptides corresponding to the immunogen, a related sequence, as well as unacetylated histone H4 peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution.
    Lane 1: AGG[acK]GG[acK]GMG[acK]VGA[acK]RHSC
    Lane 2: AGG[acK]GG[acK]GG[acK]GG[acK]GG[acK]GGC
    Lane 3: SGRGKGGKGLC
    Lane 4: GKGGAKRHRKC

for Histone H4 pan-acetyl antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

for Histone H4 pan-acetyl antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 pan-acetyl antibody.

for Histone H4 pan-acetyl antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-1 (Cat. No. 71003) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-1 (Cat. No. 71015) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4ac (pan-acetyl) antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4ac (pan-acetyl) antibody (pAb).

for Histone H4 pan-acetyl antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 pan-acetyl antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4ac (pan-acetyl) antibody (pAb)
 

Data Thumbnails

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP.

ChIP of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot.

Western blot of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)