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Histone H4ac (pan-acetyl) antibody (pAb)

商品コード: 39925 Format: 100 µg ¥69,000 Buy Now
商品コード: 39926 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H4 pan-acetyl antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
ICC/IF: 1 - 2 µg/ml dilution
WB: 1 - 2 µg/ml dilution

Published Applications:
ChIP
WB

for Histone H4 pan-acetyl antibody (pAb)Immunogen

This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).

for Histone H4 pan-acetyl antibody (pAb)Buffer

Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39243) of this antibody is also available.

for Histone H4 pan-acetyl antibody (pAb)References

 
Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq.

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify Histone H4ac (pan-acetyl) binding sites. The image shows binding across a region of chromosome 12.

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP.

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H4 pan-acetyl antibody or the equivalent amount of rabbit IgG as a negative control. Real-time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence.

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence.
HeLa cells stained at 2 µg/ml with Histone H4 pan-acetyl antibody. Top panel: Histone H4 pan-acetyl antibody. Middle panel: DAPI. Bottom panel: merge.

Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot.

Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H4 pan-acetyl (1 µg per ml).
Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.

Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl antibody for acetyl histone H4. An acetylated peptide corresponding to the immunogen (Lane 1) as well an unacetylated histone H4 peptide (Lane 2) were spotted onto PVDF and probed with Histone H4 pan-acetyl antibody at 1 µg /ml. The amount of peptide (picomoles) spotted is indicated next to each row.

for Histone H4 pan-acetyl antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

for Histone H4 pan-acetyl antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H4 pan-acetyl antibody.

for Histone H4 pan-acetyl antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4ac (pan-acetyl) antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4ac (pan-acetyl) antibody (pAb).

for Histone H4 pan-acetyl antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 pan-acetyl antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4ac (pan-acetyl) antibody (pAb)
 

Data Thumbnails

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP.

ChIP of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot.

Western blot of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)

Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4ac (pan-acetyl) pAb.
(Click image to enlarge and see details.)