Your Cart
Browse
Filter
RNA pol II CTD phospho Ser2 antibody (pAb)
| Catalog No: 39563 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39564 | Format: 10 µl | $70 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for RNA pol II CTD phospho Ser2 antibody (pAb)Background
RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.
for RNA pol II CTD phospho Ser2 antibody (pAb)Immunogen
This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at serine 2.
for RNA pol II CTD phospho Ser2 antibody (pAb)Application Notes
This RNA pol II CTD phospho Ser2 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for RNA pol II CTD phospho Ser2 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for RNA pol II CTD phospho Ser2 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Ser2 antibody.
RNA Pol II CTD phospho Ser2 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with RNA Pol II CTD phospho Ser2 pAb at a dilution of 1:2,000.
RNA Pol II CTD phospho Ser2 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of RNA Pol II CTD phospho Ser2 pAb. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Phospho Ser2 of RNA Pol II CTD peptide.
Lane 2: Unmodified RNA Pol II CTD peptide.
Lane 3: Phospho Ser5 of RNA Pol II CTD peptide.
for RNA pol II CTD phospho Ser2 antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for RNA pol II CTD phospho Ser2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
