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Recombinant HDAC4 protein

Aliases: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
商品コード: 31350 Format: 10 µg ¥73,000 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC4 proteinContents

10 µg recombinant HDAC4 supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione and 10% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC4 proteinBackground

HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

for Recombinant HDAC4 proteinApplication Notes

Recombinant HDAC4 protein is suitable for use in histone deactylase assays. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: 103,255 pmol/min/μg.

Assay conditions: Prepare 25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 μM HDAC class IIa substrate and recombinant HDAC4 protein. Incubate for 30 minutes at 37°C followed by developing for 15 minutes at room temperature.

for Recombinant HDAC4 proteinProtein Details

HDAC4 is a Class II histone deacetylase with broad specificity. The C-terminal part of HDAC4 (accession number NM_006037), amino acids 627-1084 (end), was expressed with an N-terminal GST-Tag (MW = 75.2 kDa) in a baculovirus expression system.

 
HDAC4 protein Coomassie gel

HDAC4 protein gel.
HDAC4 run on an SDS-PAGE gel and stained with Coomassie blue.

HDAC4 activity assay

HDAC4 activity assay.
Recombinant HDAC4 activity measured using a fluorescent HDAC assay.

for Recombinant HDAC4 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC4 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC4 protein
 

Data Thumbnails

HDAC4 protein Coomassie gel

HDAC4 protein Coomassie gel.
(Click image to enlarge and see details).

HDAC4 activity assay

HDAC4 activity assay.
(Click image to enlarge and see details).