Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Recombinant HDAC4 protein

Aliases: Histone Deacetylase 4, HD4; HDACA; HA6116; HDAC-4; HDAC-A
商品コード: 31364 Format: 100 µg ¥73,000 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant HDAC4 proteinContents

100 µg of recombinant HDAC4 protein expressed in E. coli and provided in 25mM HEPES pH7.5, 200mM KCl, 1mM DTT and 50% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC4 proteinBackground

HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

for Recombinant HDAC4 proteinApplication Notes

Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.

for Recombinant HDAC4 proteinProtein Details

HDAC4 is a Class II histone deacetylase with broad substrate specificity. The catalytic domain corresponding to amino acids 645 - 1057 of HDAC4 (accession number NP_006028.2) was expressed in E. coli with an apparent molecular weight of 45 kDa. An epitope tag present during expression was removed proteolytically after purification.

 
HDAC4 Coomassie gel.

HDAC4 protein gel.
HDAC4 run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant HDAC4 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC4 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant HDAC4 protein
 

Data Thumbnails

HDAC4 Coomassie gel.

HDAC4 Coomassie stained gel.
(Click image to enlarge and see details).