Bisulfite Sequencing Services
determine DNA methylation status at base pair resolution
Active Motif’s Targeted Next-Gen Bisulfite Sequencing Service offers significant advantages over the traditional Sanger-based method, most notably the ability to multiplex several amplicons from multiple bisulfite-converted DNA samples in a single Next-Gen sequencing reaction. Because 100X to 10,000X more data is generated for each cytosine, the resulting data is more statistically significant than Sanger sequencing. The service is an end-to-end assay and includes bisulfite conversion, primer design and testing, PCR amplification, library preparation, DNA sequencing and data analysis.
Advantages of Targeted Next-Gen Bisulfite Sequencing
- More sequencing data results in greater statistical significance
- Use longer amplicons than Sanger Bisulfite Sequencing
- A more cost effective option for large-scale experiments using multiple cell types and/or amplicons
Figure 1: Heat map of Targeted Next-Gen Bisulfite Sequencing data.
Figure 2: Comparison of Sanger and Targeted Next-Gen Bisulfite Sequencing data.
The papers below cite the use of and/or provide additional information about Next-Gen Bisulfite Sequencing Services provided by Active Motif’s Epigenetic Services:
- “Parental olfactory experience influences behavior and neural structure in subsequent generations” by Dias et al (2014) Nature Neuroscience 17(1):89-96.
- “Loss of NAPRT1 Expression by Tumor-Specific Promoter Methylation Provides a Novel Predictive Biomarker for NAMPT Inhibitors” by Shames et al (2013) Clinical Cancer Research 19(24):6912-6923.