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For GSDIM (Ground State Depletion with Individual Molecule return) microscopy, Leica Microsystems successfully tested Active Motif Chromeon's fluorescent ATTO 647N (STED/GSD) conjugates. The fluorescent properties of these dyes meet the specifications required to perform GSDIM microscopy with the 500 mW 642 nm laser-line of the SR GSD system. Active Motif Chromeon's Fluorescent Secondary Antibody Conjugates have been prepared using an optimized protocol that ensures the highest fluorescent intensity and stability. In addition, the ATTO dye conjugates have been maximally cross-adsorbed against IgG's of a variety of species to eliminate background caused by non-specific binding. These unique features make the Active Motif Chromeon ATTO dye secondary conjugates ideal tools for GSD microscopy, which will help in your explorations of sub-cellular space.
To ensure that you achieve the best image quality possible, ATTO 647N (STED/GSD)-stained slides should be embedded in MEA (β-Mercaptoethylamine) or Mowiol-based embedding media.
Figure 1: Active Motif Chromeon ATTO 647N (STED/GSD) antibody conjugates in widefield and GSDIM microscopy.Tubulin was stained with a primary monoclonal mouse antibody and with ATTO 647N (STED/GSD) Goat anti-mouse IgG (Catalog No. 15038) secondary antibody (left). The widefield (left) and GSDIM (right) images are courtesy of Leica Microsystems, Germany.
For additional information on ATTO antibody conjugates, please visit the Fluorescent Secondary Antibody Conjugates page.
