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6メチル化アデニン（m6A）は、ほぼすべてのRNAタイプおよび細菌からヒトへの全ての生物において広く見られ、mRNAのスプライシング、プロセシング、翻訳効率、編集およびmRNA安定性の効率において重要な役割を果たしています。 アクティブ・モティフでは、m6A RNAメチル化研究のための高品質、アッセイで検証された組換えタンパク質、ならびに抗体、アッセイキット、およびRNA修飾の分析用試薬をご提供しています。
MTase-Glo assay for METTL3 / METTL14 Complex m6A Methyltransferase Activity
1 μM Substrate RNA (UAGAGGACCAGUCGGACCAGUCGGACCGAU) and 1 μM SAM was incubated with different concentrations of METTL3 / METTL14 Complex (Cat. No. 31570) in an 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, and 1 mM TCEP at room temperature for 1 hour (0.2 U/μl RRI was added in this system). 5xMTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. An SAH standard curve (0-1 μM) was performed following the same protocol.
| Name | Expressed In | Format | Cat No. | 価格 (税抜) | |
|---|---|---|---|---|---|
| AbFlex® N6-Methyladenosine (m6A) antibody (rAb) | N/A | 100 µg | 91261 | ¥72,000 | Buy |
| 10 µg | 91262 | ¥20,000 | Buy | ||
| N6-Methyladenosine (m6A) antibody (mAb) | N/A | 100 µg | 61755 | ¥89,000 | Buy |
| 10 µg | 61756 | ¥25,000 | Buy | ||
| N6-Methyladenosine (m6A) antibody (pAb) | N/A | 100 µg | 61495 | ¥89,000 | Buy |
| 10 µg | 61496 | ¥20,000 | Buy | ||
| 50 µg | 61995 | ¥53,000 | Buy | ||
| Recombinant ALKBH1 protein | E. coli | 50 µg | 81128 | ¥99,000 | Buy |
| 1 mg | 81828 | ¥608,000 | Buy | ||
| Recombinant ALKBH2 protein | E. coli | 100 µg | 81129 | ¥99,000 | Buy |
| 1 mg | 81829 | ¥608,000 | Buy | ||
| Recombinant ALKBH3 protein | E. coli | 100 µg | 81130 | ¥99,000 | Buy |
| 1 mg | 81830 | ¥608,000 | Buy | ||
| Recombinant ALKBH4 protein | E. coli | 100 µg | 81131 | ¥99,000 | Buy |
| 1 mg | 81831 | ¥608,000 | Buy | ||
| Recombinant ALKBH5 protein | Baculovirus | 20 µg | 31589 | ¥99,000 | Buy |
| Recombinant ALKBH7 protein | E. coli | 100 µg | 81132 | ¥99,000 | Buy |
| 1 mg | 81832 | ¥608,000 | Buy | ||
| Recombinant ALKBH8 protein | E. coli | 100 µg | 81197 | ¥99,000 | Buy |
| 1 mg | 81797 | ¥608,000 | Buy |
