RNAメチル化 関連製品

m6A RNAメチル化アッセイへの応用

6メチル化アデニン(m6A)は、ほぼすべてのRNAタイプおよび細菌からヒトへの全ての生物において広く見られ、mRNAのスプライシング、プロセシング、翻訳効率、編集およびmRNA安定性の効率において重要な役割を果たしています。 アクティブ・モティフでは、m6A RNAメチル化研究のための高品質、アッセイで検証された組換えタンパク質、ならびに抗体、アッセイキット、およびRNA修飾の分析用試薬をご提供しています。

MTase-Glo assay for METTL3/METTL4 Complex m6A methyltransferase activity

MTase-Glo assay for METTL3 / METTL14 Complex m6A Methyltransferase Activity

1 μM Substrate RNA (UAGAGGACCAGUCGGACCAGUCGGACCGAU) and 1 μM SAM was incubated with different concentrations of METTL3 / METTL14 Complex (Cat. No. 31570) in an 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, and 1 mM TCEP at room temperature for 1 hour (0.2 U/μl RRI was added in this system). 5xMTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. An SAH standard curve (0-1 μM) was performed following the same protocol.

 
Name Expressed In Format Cat No. Price  
Recombinant ALKBH2 protein E. coli 100 µg 81129 ¥62,000 Buy
1 mg 81829 ¥400,000 Buy
Recombinant ALKBH3 protein E. coli 100 µg 81130 ¥50,000 Buy
1 mg 81830 ¥400,000 Buy
Recombinant ALKBH4 protein E. coli 100 µg 81131 ¥76,000 Buy
1 mg 81831 ¥481,000 Buy
Recombinant ALKBH5 protein Baculovirus 20 µg 31589 ¥78,000 Buy
Recombinant ALKBH6 protein E. coli 50 µg 81191 ¥76,000 Buy
1 mg 81891 ¥481,000 Buy
Recombinant ALKBH7 protein E. coli 100 µg 81132 ¥76,000 Buy
1 mg 81832 ¥481,000 Buy
Recombinant ALKBH8 protein E. coli 100 µg 81197 ¥76,000 Buy
1 mg 81797 ¥481,000 Buy
Recombinant FTO protein Baculovirus 20 µg 31572 ¥76,000 Buy
1 mg 31972 ¥648,000 Buy
Recombinant FTSJ3 protein Baculovirus 20 µg 81189 ¥75,000 Buy
1 mg 81889 ¥648,000 Buy
Recombinant METTL1 protein Baculovirus 20 µg 81022 ¥76,000 Buy