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リコンビナントタンパク質・酵素

RNAメチル化 関連製品

m6A RNAメチル化アッセイへの応用

6メチル化アデニン(m6A)は、ほぼすべてのRNAタイプおよび細菌からヒトへの全ての生物において広く見られ、mRNAのスプライシング、プロセシング、翻訳効率、編集およびmRNA安定性の効率において重要な役割を果たしています。 アクティブ/モティーフでは、m6A RNAメチル化研究のための高品質、アッセイで検証された組換えタンパク質、ならびに抗体、アッセイキット、およびRNA修飾の分析用試薬をご提供しています。

MTase-Glo assay for METTL3/METTL4 Complex m6A methyltransferase activity

MTase-Glo assay for METTL3 / METTL14 Complex m6A Methyltransferase Activity

1 μM Substrate RNA (UAGAGGACCAGUCGGACCAGUCGGACCGAU) and 1 μM SAM was incubated with different concentrations of METTL3 / METTL14 Complex (Cat. No. 31570) in an 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, and 1 mM TCEP at room temperature for 1 hour (0.2 U/μl RRI was added in this system). 5xMTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. An SAH standard curve (0-1 μM) was performed following the same protocol.

 
Name Expressed In Format Cat No. 価格 (税抜)  
Recombinant ALKBH2 protein E. coli 100 µg 81129 ¥73,000 Buy Now
1 mg 81829 ¥481,000 Buy Now
Recombinant ALKBH3 protein E. coli 100 µg 81130 ¥73,000 Buy Now
1 mg 81830 ¥481,000 Buy Now
Recombinant ALKBH4 protein E. coli 100 µg 81131 ¥73,000 Buy Now
1 mg 81831 ¥481,000 Buy Now
Recombinant ALKBH5 protein Baculovirus 20 µg 31589 ¥74,000 Buy Now
Recombinant ALKBH6 protein E. coli 50 µg 81191 ¥73,000 Buy Now
1 mg 81891 ¥481,000 Buy Now
Recombinant ALKBH7 protein E. coli 100 µg 81132 ¥73,000 Buy Now
1 mg 81832 ¥481,000 Buy Now
Recombinant ALKBH8 protein E. coli 100 µg 81197 ¥73,000 Buy Now
1 mg 81797 ¥481,000 Buy Now
Recombinant FTO protein Baculovirus 20 µg 31572 ¥74,000 Buy Now
1 mg 31972 ¥648,000 Buy Now
Recombinant METTL1 protein Baculovirus 20 µg 81022 ¥74,000 Buy Now
Recombinant METTL1 protein, GST-Tag E. coli 100 µg 81059 ¥73,000 Buy Now
1 mg 81759 ¥481,000 Buy Now
Recombinant METTL1 protein, His-Tag E. coli 100 µg 81058 ¥73,000 Buy Now
1 mg 81758 ¥481,000 Buy Now
Recombinant METTL2A protein Baculovirus 20 µg 81027 ¥74,000 Buy Now
Recombinant METTL3 protein Baculovirus 20 µg 31567 ¥74,000 Buy Now
Recombinant METTL3 / METTL14 complex Baculovirus 20 µg 31570 ¥77,000 Buy Now
Recombinant METTL6 protein Baculovirus 20 µg 81023 ¥74,000 Buy Now
Recombinant METTL6 protein, GST-Tag E. coli 100 µg 81061 ¥73,000 Buy Now
1 mg 81761 ¥481,000 Buy Now
Recombinant METTL6 protein, His-Tag E. coli 100 µg 81060 ¥73,000 Buy Now
1 mg 81760 ¥481,000 Buy Now
Recombinant METTL8 protein Baculovirus 20 µg 81028 ¥74,000 Buy Now
Recombinant METTL8 protein, GST-Tag E. coli 50 µg 81106 ¥73,000 Buy Now
1 mg 81806 ¥481,000 Buy Now
Recombinant METTL11A (NTMT1) protein Baculovirus 20 µg 81029 ¥73,000 Buy Now
1 mg 81729 ¥648,000 Buy Now
Recombinant METTL11A (NTMT1) protein, His-Tag E. coli 100 µg 81062 ¥73,000 Buy Now
1 mg 81762 ¥481,000 Buy Now
Recombinant METTL11B (NTM1B) protein E. coli 50 µg 81121 ¥73,000 Buy Now
1 mg 81821 ¥481,000 Buy Now
Recombinant METTL13 protein Baculovirus 20 µg 81024 ¥74,000 Buy Now
Recombinant METTL14 protein Baculovirus 20 µg 31568 ¥74,000 Buy Now
Recombinant METTL15 protein Baculovirus 20 µg 81084 ¥73,000 Buy Now
1 mg 81784 ¥648,000 Buy Now
Recombinant METTL16 protein Baculovirus 20 µg 81085 ¥73,000 Buy Now
1 mg 81785 ¥648,000 Buy Now
Recombinant METTL17 protein Baculovirus 10 µg 81105 ¥73,000 Buy Now
1 mg 81805 ¥1,091,500 Buy Now
Recombinant METTL18 protein Baculovirus 20 µg 81030 ¥74,000 Buy Now
Recombinant METTL19 (TRMT44) protein Baculovirus 20 µg 81088 ¥73,000 Buy Now
1 mg 81788 ¥648,000 Buy Now
Recombinant METTL21A protein E. coli 100 µg 81122 ¥73,000 Buy Now
1 mg 81822 ¥481,000 Buy Now
Recombinant METTL21C protein E. coli 50 µg 81123 ¥73,000 Buy Now
1 mg 81823 ¥481,000 Buy Now
Recombinant METTL22 protein Baculovirus 20 µg 81092 ¥73,000 Buy Now
1 mg 81892 ¥648,000 Buy Now
Recombinant METTL25 protein Baculovirus 20 µg 81089 ¥73,000 Buy Now
1 mg 81789 ¥648,000 Buy Now
Recombinant METTL26 (JFP2) protein E. coli 100 µg 81063 ¥73,000 Buy Now
1 mg 81763 ¥481,000 Buy Now
Recombinant NSUN1 protein Baculovirus 20 µg 81170 ¥75,000 Buy Now
1 mg 81870 ¥648,000 Buy Now
Recombinant NSUN2 protein Baculovirus 20 µg 81171 ¥75,000 Buy Now
1 mg 81871 ¥648,000 Buy Now
Recombinant NSUN3 protein Baculovirus 20 µg 81183 ¥75,000 Buy Now
Recombinant NSUN4 (SHTAP) protein Baculovirus 20 µg 81172 ¥75,000 Buy Now
1 mg 81872 ¥648,000 Buy Now
Recombinant NSUN5 protein Baculovirus 20 µg 81173 ¥75,000 Buy Now
1 mg 81873 ¥648,000 Buy Now
Recombinant NSUN6 protein Baculovirus 20 µg 81174 ¥75,000 Buy Now
1 mg 81874 ¥648,000 Buy Now
Recombinant NSUN7 protein Baculovirus 10 µg 81175 ¥75,000 Buy Now
Recombinant WTAP protein Baculovirus 20 µg 31571 ¥74,000 Buy Now
Recombinant YTHDC2 protein Baculovirus 20 µg 81198 ¥75,000 Buy Now
Recombinant YTHDF1 protein Baculovirus 20 µg 31608 ¥74,000 Buy Now
Recombinant YTHDF2 protein Baculovirus 20 µg 31573 ¥74,000 Buy Now
Recombinant YTHDF3 (407-560) protein E. coli 100 µg 81104 ¥73,000 Buy Now
1 mg 81804 ¥481,000 Buy Now