ChIP 101 eBook: A Beginner’s Guide to Performing Chromatin Immunoprecipitation Assays
The chromatin immunoprecipitation (ChIP) assay is a powerful method to study protein-DNA interactions and to investigate the locations of histone modifications across the genome. But ChIP assays take time to master. It’s also challenging to know which antibody will work best for your target protein and which protocol is best for your sample type.
This free eBook makes it easy to become a ChIP assay expert by covering the main steps in the ChIP workflow and giving pointers on how to achieve the best results.
Topics covered include:
- The 5 critical steps in every ChIP experiment
- How many cells are needed for ChIP assays?
- How to properly fix and prepare your samples for ChIP
- How to sonicate and solubilize chromatin
- The best type of antibody to use in ChIP assays
- The best ways to analyze ChIP results