Global DNA Methylation Assays

sensitive, specific ELISA-based assays for analysis of DNA methylation states

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Active Motif offers easy-to-use, high-throughput assays for quantification of global levels of DNA methylation (5-mC) and hydroxymethylation (5-hmC). Variances in global DNA methylation and hydroxymethylation have been observed across different developmental states and tissue types, and changes in global methylation have also been associated with various diseases, including cancer. Common methods used to measure global DNA methylation levels include chromatographic methods such as HPLC and mass spectrometry. However, these methods require a high level of expertise, are low throughput and time-consuming. Furthermore, they are not able to effectively distinguish between 5-mC and 5-hmC. Active Motif makes it easier more cost-effective to measure these changes by providing highly sensitive and specific plate-based ELISA-based assays as an alternative to traditional chromatographic techniques. The stripwell microplate formats enable scalability for manual or high throughput analysis. The assays use antibodies that are highly specific for their target DNA modifications, ensuring there is no cross-reactivity with other DNA methyl variants. All kits include DNA standards to enable quantification.