For GSDIM (Ground State Depletion with Individual Molecule return) microscopy using the Leica SR GSD microscope, the use of fluorescent ATTO 532 dyes are strongly recommended by Leica Microsystems. The fluorescent properties of these dyes meet the specifications required to perform GSDIM microscopy with the 500 mW 532 nm laser-line of the SR GSD system. Active Motif's Fluorescent Secondary Antibody Conjugates have been prepared using an optimized protocol that ensures the highest fluorescent intensity and stability. In addition, the ATTO dye conjugates have been maximally cross-adsorbed against IgG's of a variety of species to eliminate background caused by non-specific binding. These unique features make the Active Motif ATTO dye secondary conjugates ideal tools for GSD microscopy, which will help in your explorations of sub-cellular space.
To ensure that you achieve the best image quality possible, ATTO 532 (GSD)-stained slides should be embedded in either Glucose-Oxidase Mix (containing glucose, glucose oxidase and catalase in a PBS buffer of pH = 7.4) or PVA (Polyvinyl alcohol) -based embedding media.
Figure 1: Active Motif ATTO 532 (GSD) antibody conjugates in widefield and GSDIM microscopy.