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CUT&Tag (Cleavage Under Targets and Tagmentation) は、protein A (pA) とTn5のキメラ (pA-Tn5) と、解析したいヒストンまたは修飾ヒストンに対するマウスまたはウサギ一次抗体を用いて、ゲノムワイドにターゲット周辺のDNAをタグ付けし、それらの遺伝子配列を明らかにする解析法です。pA-Tn5を使用することで、クロマチンの断片化などのステップを経ずにNGSライブラリー調製ができるため、CUT&Tagは、ChIP-Seqよりもはるかに少ないサンプルから短時間 (1-2日) かつ深度の浅いシーケンスで十分な解析を可能にします。
Analyze CUT&Tag-IT R-loop Assay datasets confidently to detect true biological differences.
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