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Cleavage Under Target and Tagmentation (CUT&Tag)は、従来のChIPアッセイに代わる手法で、各種ヒストン修飾あるいは他のタンパク質とDNA間の相互作用を明らかにしたり、標的タンパク質のDNA結合部位を同定したりすることができます。CUT&Tagでは、標的タンパク質に対する特異的な一次抗体を必要としますが、そのタンパク質を未固定のネイティブな状態で特異的に検出しなくてはなりません。アクティブ・モティフは、ヒストンやヒストン修飾、クロマチンタンパク質の他、多様な因子に対する高品質な抗体を製造しており、CUT&Tagアッセイにおいて良好な結果がえられることを検証した抗体も数多く取り揃えています。今後、CUT＆Tagに適用可能な抗体を拡充していきます。
Active Motif Antibodies Published for Use in CUT&Tag
The following applications have been published using these antibodies and is provided for reference. Active Motif may not have validated these antibodies for use in these applications.
CUT&Tag Publications
下の表に含まれる抗体はCUT&Tag-IT® Assay Kitsを用いて使用可能なことを検証しています。各製品の詳細については抗体名をクリックしてください。
| Name | Isotype | Reactivity | Appl. | Format | Cat No. | 価格 (税抜) | |
|---|---|---|---|---|---|---|---|
| AbFlex® CTCF antibody (rAb) | IgG | Human | ChIP, ChIP-Seq, CUT&Tag, WB | 100 µg | 91285 | ¥72,000 | Buy |
| 10 µg | 91286 | ¥20,000 | Buy | ||||
| Histone H2A.Z antibody (pAb) | Serum | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, IF, WB | 100 µl | 39113 | ¥92,000 | Buy |
| 50 µl | 39013 | ¥55,000 | Buy | ||||
| 10 µl | 39114 | ¥25,000 | Buy | ||||
| Histone H3K4me1 antibody (mAb) | IgG2b | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, WB | 100 µg | 39635 | ¥92,000 | Buy |
| AbFlex® Histone H3K4me1 antibody (rAb) | IgG | Human, Wide Range Predicted | CUT&Tag, WB | 100 µg | 91415 | ¥72,000 | Buy |
| 10 µg | 91416 | ¥20,000 | Buy | ||||
| Histone H3K4me2 antibody (pAb) | Serum | Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, WB | 100 µl | 39141 | ¥92,000 | Buy |
| 50 µl | 39042 | ¥55,000 | Buy | ||||
| 10 µl | 39142 | ¥25,000 | Buy | ||||
| AbFlex® Histone H3K4me3 antibody (rAb) | IgG2a | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, WB | 100 µg | 91263 | ¥72,000 | Buy |
| 10 µg | 91264 | ¥20,000 | Buy | ||||
| Histone H3K4me3 antibody (pAb) | Serum | Budding Yeast, Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, IHC, WB | 100 µl | 39159 | ¥92,000 | Buy |
| 50 µl | 39060 | ¥55,000 | Buy | ||||
| 10 µl | 39160 | ¥25,000 | Buy | ||||
| Histone H3K4me3 antibody (pAb) | IgG | Budding Yeast, Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, WB | 100 µg | 39915 | ¥92,000 | Buy |
| 50 µg | 39016 | ¥55,000 | Buy | ||||
| 10 µg | 39916 | ¥25,000 | Buy | ||||
| Histone H3K9ac antibody (pAb) | IgG | Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, ICC, IF, WB | 100 µg | 39917 | ¥92,000 | Buy |
| 50 µg | 39017 | ¥55,000 | Buy | ||||
| 10 µg | 39918 | ¥25,000 | Buy | ||||
| Histone H3K9me3 antibody (pAb) | Serum | Fission Yeast, Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, ICC, IF, IHC, WB | 100 µl | 39161 | ¥92,000 | Buy |
| 50 µl | 39062 | ¥55,000 | Buy | ||||
| 10 µl | 39162 | ¥25,000 | Buy |
