ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells (Jason Buenrostro)
July 22, 2021
In this episode of the Epigenetics Podcast, we caught up with Jason Buenrostro from Harvard University to talk about his work on developing biological tools to measure chromatin dynamics in single-cells.
We hear the story of how in his early years in the lab, Jason took a risk and added an enzyme called Transposase to cells in culture. What he saw on an agarose gel astonished him. He was able to recreate a nucleosomal ladder similar to using MNase or DNase-Seq, without the tedious steps of optimization. In following years he optimized the method into what is known today as ATAC-Seq. In recent years he was also able to bring ATAC-Seq to the next level, developing single cell ATAC-Seq (scATAC-Seq), and combining it with RNA-Seq in a multi-omics approach.
In this Episode we discuss how Jason Buenrostro developed ATAC-Seq in William Greenleaf's lab, how a lack of equipment shaped the ATAC-Seq protocol, and how scATAC-Seq has enabled a whole different way of looking at biological samples.