Recombinant KDM1B / LSD2 protein
|Aliases: Lysine Demethylase 1B, Flavin-Containing Amine Oxidase Domain-Containing Protein 1|
|Catalog No: 31479||Format: 20 µg||¥74,000||Buy|
|Catalog No: 31879||Format: 1 mg||¥592,000||Buy|
Full length recombinant KDM1B / LSD2 protein containing an N-terminal GST-Tag expressed in Sf9 cells and supplied in 25 mM HEPES, pH 7.5, 120 mM NaCl 20 mM GSH and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.
KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (by similarity).
Recombinant KDM1B / LSD2 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Specific Activity: H3K4me2 and H3K4me1 demethylase.
Catalytic Ability: > 600 turnovers / enzyme molecule
Histone Demethylase Assay Conditions: 3.3 μM H3K4me2 (1-27aa) peptide was incubated with different concentrations of KDM1B / LSD2 in reaction buffer containing 50 mM TrisCl, pH 8.0, 0.02% Triton X-100, 50 nM FAD for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection.
Full-length Recombinant KDM1B / LSD2 (accession number XM_005248926.1) was expressed in was expressed in Sf9 cells and contains an N-terminal GST-Tag with an observed molecular weight of 116 kDa. The recombinant protein is >92% pure by SDS-PAGE.
This product was used in the following publications:
J. Biol. Chem. (2016). 291(47): 24756-24767. PMID: 27634040.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.