- 受託サービス
- 機能ゲノミクス - LightSwitch™
- 遺伝子発現制御
-
エピジェネティクス・クロマチン
- クロマチン再構築
- クロマチン免疫沈降 (ChIP)
- DNAメチル化解析
-
ヒストン解析
- HATアッセイ(ヒストンアセチルトランスフェラーゼ)
- ヒストン脱アセチル化酵素活性(HDAC)
-
Histone Modification ELISAs
- Total Histone H3 ELISA
- Histone H3 methylated Lys4 ELISA (H3K4)
- Histone H3 acetyl Lys9 ELISA (H3K9)
- Histone H3 methylated Lys9 ELISA (H3K9)
- Histone H3 acetyl Lys14 ELISA (H3K14)
- Histone H3 acetyl Lys27 ELISA (H3K27)
- Histone H3 methylated Lys27 ELISA (H3K27)
- Histone H3 phospho Ser10 ELISA (H3S10ph)
- Histone H3 phospho Ser28 ELISA (H3S28ph)
- Histone Peptide Array
- ヒストン単離
- 組換えヒストンタンパク質
- ヌクレオソーム解析
- 活性化物質 / 阻害物質
- 抗体
- 抗体標識
- リコンビナントタンパク質・酵素
- タンパク質検出・定量
- 細胞・組織・酸抽出物
- 蛍光検出・標識
- サンプル調製
- 形質転換・遺伝子発現
- バイオインフォマティクス
- Fluorescent Dyes and Antibodies
- クリックケミストリー用蛍光ツール
- MAX Stain™ Immunofluorescence Tools
- Super-Resolution Fluorescence Microscopy
- Fluorescent Kits for Cell Biology
-
(871)
Antibodies
- Antibody Type
- Application
- Isotype
- Reactivity
- Modification
- Purification
- Host
- Product Category
- Histone Products
- New Products
- Sample Available
- Antibody Category
-
Research Area
- (464) Chromatin & Histones
- (426) Transcription
- (179) Methylation
- (103) Acetylation & Deacetylation
- (101) Phosphorylation
- (78) Cell Cycle
- (71) DNA Damage & Repair
- (68) Cell Signaling
- (43) Apoptosis
- (21) Nuclear Receptors
- (18) Fluorescence
- (6) Oxidative Stress
- (4) Post Transcription
- (3) Immunoprecipitation
- (1) ELISAs
- (1) SUMOylation
- (1) Ubiquitination
- More
-
(99)
Extracts
- Extract Type
- Extract Species
-
Treatment
- (62) Untreated
- (6) TPA
- (3) IL-6
- (2) Forskolin
- (2) H2O2
- (2) Serum
- (2) TNF-α
- (2) TPA + CI
- (1) Acetaldehyde
- (1) Anacardic acid
- (1) Anisomycin
- (1) CoCl2
- (1) Colcemid
- (1) EGF
- (1) Estradiol
- (1) Etoposide
- (1) Heat shock
- (1) Hypoxia
- (1) IFNγ
- (1) IL-1α
- (1) PHA
- (1) Paclitaxel
- (1) Prolactin
- (1) Retinoic acid
- (1) Sodium butyrate
- (1) Testosterone
- More
-
Cell Line / Tissue
- (18) HeLa
- (7) Hep G2
- (6) Jurkat
- (4) MCF-7
- (4) PC-12
- (3) COS-7
- (3) NIH/3T3
- (3) U-937
- (3) WI-38
- (2) A-431
- (2) C2C12
- (2) F9
- (2) K-562
- (2) LNCaP
- (2) Nb2
- (2) SL-2
- (2) T-47D
- (2) THP-1
- (1) 293
- (1) 3T3-L1
- (1) CCRM-CEM
- (1) Caco-2
- (1) DU 145
- (1) HL-60
- (1) HT-29
- (1) Human Heart
- (1) Human Liver
- (1) Human Lung
- (1) JEG-3
- (1) KG-1
- (1) LoVo
- (1) MDA-MB-231
- (1) MG-63
- (1) MIA PaCa-2
- (1) Mouse Brain
- (1) NIH:OVCAR-3
- (1) P19
- (1) PANC-1
- (1) PC-03
- (1) Raji
- (1) Rat Brain
- (1) Rat Liver
- (1) SK-N-BE
- (1) SW48
- (1) Saos-2
- (1) U-87
- (1) Y-79
- (1) ZR-75-1
- More
- Research Area
- Histone Products
- Modification
- (25) Fluorescent Dyes
-
(326)
Kits
- New Products
-
Product Category
- (94) Chromatin Immunoprecipitation
- (48) Phospho-protein Detection
- (44) Transcription Factor ELISAs
- (42) Sample Preparation
- (25) Epigenetic Services
- (14) DNA Methylation
- (14) Histone ELISAs
- (10) Gelshift / Supershift
- (8) Histone Assays
- (7) Antibody Labeling
- (4) Co-Immunoprecipitation
- (4) Immunofluorescence
- (3) Histone Array
- (2) Chromatin Assembly
- (2) Mitosis Assays
- (1) Nitric Oxide Assays
- (1) Nucleosomes
- (1) Protein Delivery
- (1) Ras GTPase
- (1) miRNA Target Validation
- More
-
Research Area
- (176) Chromatin & Histones
- (114) Transcription
- (102) ELISAs
- (100) Immunoprecipitation
- (93) Accessories
- (78) Sample Preparation
- (77) Phosphorylation
- (60) Cell Signaling
- (48) Methylation
- (23) Cell Cycle
- (20) Apoptosis
- (16) Acetylation & Deacetylation
- (15) Sonication
- (10) DNA Damage & Repair
- (8) Fluorescence
- (3) Nuclear Receptors
- (3) Oxidative Stress
- (2) Gene Regulation
- (1) Clinical Research
- (1) DNA Methylation
- (1) Histone Post Translational Modifications
- (1) Post Transcription
- More
- Histone Products
- Modification
- Product Class
-
(599)
Recombinant Proteins
- New Products
-
Protein Category
- (201) Histone
- (158) Transcription
- (57) Bromodomain
- (55) Histone Methyltransferase (HMT)
- (25) Kinase
- (24) Histone Deacetylase (HDAC)
- (20) Histone Demethylase (HDM)
- (18) DNA Damage
- (16) Nuclear Receptor
- (10) Histone Acetyltransferase (HAT)
- (9) DNA Methyltransferase (DNMT)
- (5) Cytosine Oxygenase (TET)
- (1) Glucosyltransferase
- (1) Neddylation
- (1) Ubiquitination
- More
- Expressed In
- Protein Species
- Modification
-
Research Area
- (385) Chromatin & Histones
- (239) Methylation
- (201) Transcription
- (127) Acetylation & Deacetylation
- (43) Phosphorylation
- (39) Cell Signaling
- (25) Post Transcription
- (21) Cell Cycle
- (16) Nuclear Receptors
- (14) DNA Damage & Repair
- (12) Oxidative Stress
- (9) Apoptosis
- (3) Ubiquitination
- (2) Sample Preparation
- More
- Histone Products
-
(149)
Reporter Assays
- LightSwitch™ Collections
-
Product Category
- (92) Synthetic Response Elements
- (11) Pathway Biomarker Sets
- (8) LightSwitch™ 3´UTR Controls
- (8) LightSwitch™ Promoter Controls
- (8) LightSwitch™ Reporter Collections
- (8) Stable Reporter Cell Lines
- (4) LightSwitch™ Empty Vectors
- (3) Gene Regulation Services
- (3) Transfection Reagents
- (2) miRNA Mimics and Inhibitors
- (1) LightSwitch™ Assay Kits
- (1) Synthetic miRNA Targets
- More
- Research Area
- miRNA Products
- Type of Control
- (59) Small Molecules
抗体

- 抗体
- »
- Primary Antibodies
- »
- LSD1 / KDM1A antibody (pAb)
LSD1 / KDM1A antibody (pAb)
RRID: AB_2793175 |
Aliases: AOF2, KDM1A, BHC110 |
Catalog No: 39186 | Format: 100 µl | ¥70,000 | Buy |
Applications



Application Notes
Validated Applications:
ChIP: 10 µl per ChIP
WB: 1:500 - 1:2,000 dilution
Published Applications:
IP
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Immunogen
This antibody was raised against full-length recombinant human LSD1 / KDM1A protein.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
References
LSD1 / KDM1A antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from a human small lung cancer cell line (treated with the LSD1 inhibitor, ORY-1001, 1nM for 96 hours) and 5 µl of LSD1 / KDM1A antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
LSD1 / KDM1A antibody (pAb) tested by Western blot
Nuclear extract of HeLa cells (30 µg per lane) probed with LSD1 / KDM1A antibody (1:2,000 dilution).
Background
LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for LSD1 / KDM1A antibody.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with LSD1 / KDM1A antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting
RELATED PRODUCTS
Technical Data Sheet
LSD1 / KDM1A antibody (pAb)Data Thumbnails
ChIP of LSD1 / KDM1A pAb.
(Click image to enlarge and see details.)
Western blot of LSD1 / KDM1A pAb.
(Click image to enlarge and see details.)