Cart
Products
Diversi-Phi Indexed PhiX Overview
Related Products:
- CUT&Tag-IT® Express Assay Kit: Less variable and higher-throughput genome-wide profiling of histone marks
- ChIC/CUT&RUN Assay Kit: Genome-wide chromatin-associated protein profiling from 5,000 to 500,000 cells
- ATAC-Seq Assay Kit: Profile open chromatin regions in fresh or frozen cells or tissues
- ChIP-IT High Sensitivity®: ChIP for limited sample material and low abundance target proteins
Increasing index base diversity improves sequencing accuracy and cluster identification on Illumina platforms—especially for low-complexity libraries. It serves as a technical constant to monitor sequencing quality and enhance nucleotide diversity and is particularly beneficial for low-complexity libraries.
Diversi-Phi Indexed PhiX is a non-denatured double-stranded DNA PhiX library with all combinations of 8 x 8 bp indexes provided in 10 nMol in 10 mM Tris pH8.0 (10µl). Because Diversi-Phi Indexed PhiX is dual-indexed, it will diversify Read 1, Index 1, Index 2, Read 2.
The index sequences are designed with Hamming distance compatibility so that one or two misread bases will not convert them into another index. The benefits of this color balance in sequencing are:
- Prevents misassignment of samples: barcode errors caused by sequencing mistakes can be detected and corrected, keeping samples correctly separated in the run.
- Error tolerance: the Hamming distance allows for reliable correction of single-base sequencing errors.
- Reduces crosstalk: accidental cross-identification of samples is reduced, improving data quality.
Incorporating Diversi-Phi Indexed PhiX into Illumina sequencing runs helps ensure multiplexed samples produce accurate, reliable sequencing results.
Key Features:
- Ready-to-Use Format: Supplied as a 10 nM double-stranded DNA library in Tris buffer.
- Dual-Indexed Barcodes: Dual-index sequences compatible with Illumina systems, facilitating accurate demultiplexing and minimizing index hopping.
- High Diversity: Contains all combinations 8 x 8 bp indexes and is free of adapter dimers, making it suitable for supplementing low-diversity sequencing runs.
Why sequencing diversity matters
Sequencing diversity (or nucleotide diversity) refers to the proportion of AGCT detected in every cycle of a sequencing run. Each base is read as a different color (or color combination, depending on the sequencing chemistry), so if there is too much of one color, detection will not be accurate or even possible. Including Diversi-Phi Indexed PhiX in libraries will increase the diversity to avoid sequencing detection errors.
Usage Guidelines:
Sequencing Recommendations:
- 2-5% for a typical pool with decent diversity (ATAC, CUT&Tag, CUT&RUN, ChIP-Seq)
- Download the library pool dilution calculator for easy calculations
Diversi-Phi Indexed PhiX Contents
Diversi-Phi Indexed PhiX, store at -20°C
Diversi-Phi Indexed PhiX Documents
You might also be interested in:
| Name | Format | Cat No. | 価格 (税抜) | |
|---|---|---|---|---|
| Diversi-Phi Indexed PhiX | 1 vial | 53225 | ¥23,000 | Buy |
