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ASH2L antibody (pAb)

Aliases: ASH2, Bre2, ASH2L1, ASH2L2
商品コード: 39099 Format: 100 µl ¥70,000 Buy Now
商品コード: 39100 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Molecular Weight:77 kDa
Reactivity:Human, Other (Wide Range)


ChIP Validated ChIP-Seq Validated Western Blot Validated

for ASH2L antibody (pAb)Application Notes

Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 - 1:2,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:

See references for more information. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts.

for ASH2L antibody (pAb)Immunogen

This ASH2L antibody was raised against full-length recombinant human ASH2L protein.

for ASH2L antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ASH2L antibody (pAb)References

ASH2L antibody (pAb) tested by ChIP-Seq.

ASH2L antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify ASH2L binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

ASH2L antibody (pAb) tested by Western blot.

ASH2L pAb tested by Western blot.
ASH2L detection by Western blot. The analysis of ASH2L was performed using HeLa nuclear extract and ASH2L pAb at a 1:2,000 dilution.

for ASH2L antibody (pAb)Background

ASH2L (absent, small or homeotic discs 2) is a trithorax protein and is a component of different protein complexes, including i.e. mixed-lineage leukemia (MLL) oncoproteins, which contain histone methyltransferase activity. These complexes trimethylate the lysine 4 of histone H3. Histone H3 trimethyl Lys4 is one of histone modifications that are part of elaborate mechanisms that evolved to control the structure of the chromatin.

for ASH2L antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ASH2L antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with ASH2L antibody (pAb).

for ASH2L antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ASH2L antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

ASH2L antibody (pAb)

Data Thumbnails

ASH2L antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of ASH2L pAb.
(Click image to enlarge and see details.)

ASH2L antibody (pAb) tested by Western blot.

Western blot of ASH2L pAb.
(Click image to enlarge and see details.)