Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

Histone H1.0 antibody (pAb)

商品コード: 61417 Format: 100 µl ¥68,400 Buy Now
商品コード: 61418 Format: 10 µl ¥20,000 Buy Now

Request a quote for a bulk orderBulk Order Button

Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:30 kDa
Reactivity:Human, Mouse


Western Blot Validated

for Histone H1.0 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for Histone H1.0 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human Histone H1.0.

for Histone H1.0 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for Histone H1.0 antibody (pAb)References

Histone H1.0 antibody (pAb) tested by Western blot.

Histone H1.0 (pAb) tested by Western blot.
Nuclear Extract of 3T3-L1 cells (20 µg) following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) were probed with Histone H1.0 (pAb) at a dilution of 1:500.
Lane 1: Recombinant human Histone H1.0 (100 ng).
Lane 2: Day 0 - preadipocytes.
Lane 3: Day 12 - fully differentiated adipocytes.

for Histone H1.0 antibody (pAb)Background

Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Histone H1.0 is a unique variant considered a replacement linker histone which is expressed and incorporated into chromatin in the absence of DNA replication. Histone H1.0 is more highly expressed in cells that are growth inhibited or terminally differentiated.

for Histone H1.0 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H1.0 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Histone H1.0 antibody (pAb)

Data Thumbnails

Histone H1.0 antibody (pAb) tested by Western blot.

Western blot of Histone H1.0 pAb.
(Click image to enlarge and see details.)