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Tet1 antibody (pAb)

Aliases: LCX, Cxxc6, AA517754, BB001228, mKIAA1676, D10Ertd17e, 2510010B09Rik
商品コード: 61443 Format: 100 µl ¥70,000 Buy Now
商品コード: 61444 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:215 kDa
Reactivity:Human, Mouse


ChIP Validated ChIP-Seq Validated Western Blot Validated Immunohistochemistry Validated

for Tet1 antibody (pAb)Application Notes

Validated Applications:
ChIP: 4 µl per ChIP
ChIP-Seq: 4 µl each

Published Applications:

for Tet1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse Tet1.

for Tet1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Tet1 antibody (pAb)References

Tet1 antibody (pAb) tested by ChIP-Seq.

Tet1 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of chromatin from a human testicle and 4 µl of TET1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify TET1 binding sites. TET1 ChIP-Seq data shows the expected enrichment of TET1 at CpG islands.

Tet1 antibody (pAb) tested by Western blot.

Tet1 antibody (pAb) tested by Western blot
The analysis was performed using 40 µg of mouse ES (embryonic stem) cell nuclear extract and Tet1 antibody at 1:500. An specific band is detected at 215 kDa.

for Tet1 antibody (pAb)Background

Tet1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Tet1 antibody (pAb).

for Tet1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Tet1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Tet1 antibody (pAb)

Data Thumbnails

Tet1 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Tet1 pAb.
(Click image to enlarge and see details.)

Tet1 antibody (pAb) tested by Western blot.

Western blot of Tet1 pAb.
(Click image to enlarge and see details.)