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Histone H4K8ac antibody (pAb)

商品コード: 39171 Format: 200 µl ¥68,400 Buy Now
商品コード: 39172 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)


Western Blot Validated

for Histone H4 acetyl Lys8 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:4,000 dilution

Published Applications:

For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for Histone H4 acetyl Lys8 antibody (pAb)Immunogen

This Histone H4 acetyl Lys8 antibody was raised against a peptide including acetyl-lysine 8 of histone H4.

for Histone H4 acetyl Lys8 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

for Histone H4 acetyl Lys8 antibody (pAb)References

Histone H4K8ac antibody (pAb) tested by Western blot.

Histone H4 acetyl Lys8 pAb tested by Western blot.
Detection of acetylated Histone H4 by Western blot analysis using 10 µg HeLa acid extracts and Histone H4 acetyl Lys8 pAb at a 1:1,000 dilution.
    Lane 1: Acid extract of untreated HeLa cells.
    Lane 2: Acid extract of HeLa cells treated with sodium butyrate.

for Histone H4 acetyl Lys8 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The chromatin-remodeling complex SWI/SNF is recruited to promoters through the interaction of the bromodomain of the protein Brg-1, belonging to the SWI/SNF complex, and CBP-acetylated histone H4 Lysine 8, leading to a chromatin remodeling.

for Histone H4 acetyl Lys8 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys8 antibody.

for Histone H4 acetyl Lys8 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 acetyl Lys8 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Histone H4K8ac antibody (pAb)

Data Thumbnails

Histone H4K8ac antibody (pAb) tested by Western blot.

Western blot of Histone H4K8ac pAb.
(Click image to enlarge and see details.)