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RbAp46/48 antibody (pAb)

Catalog No: 39198 Format: 100 µl ¥68,400 Buy Now
Catalog No: 39199 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Molecular Weight:46 and 48 kDa
Reactivity:Human, Other (Wide Range)


ChIP Validated Western Blot Validated

for RbAp46/48 antibody (pAb)Application Notes

Validated Applications:
ChIP: 4 µl per ChIP
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for RbAp46/48 antibody (pAb)Immunogen

This RbAp46/48 antibody was raised against recombinant human RbAp46 protein.

for RbAp46/48 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RbAp46/48 antibody (pAb)References

RbAp46/48 antibody (pAb) tested by ChIP.

RbAp46/48 antibody (pAb) tested by ChIP
ChIP performed on MCF7 cell chromatin (40 μg) using 39198. PCR was performed using primers specific for the promoter region of the human SNAIL gene. Lane 1: Input DNA control. Lane 2: ChIP using 4 μl of 39198. Lane 3: Duplicate. Lane 4: Rabbit IgG as control (-). Lane 5: Duplicate. Lane 6: PCR mix.

RbAp46/48 antibody (pAb) tested by Western blot.

RbAp46/48 pAb tested by Western blot.
HeLa cell nuclear extract (10 µg per lane) was probed with RbAp46/48 pAb (1:500 dilution).

for RbAp46/48 antibody (pAb)Background

RbAp46 (Retinoblastoma protein associated protein 46) and RbAp48 (Retinoblastoma protein associated protein 48) are highly homologous histone chaperones that play key roles in protein complexes that function to establish and maintain chromatin structure. RbAp46/48 are found in a number of protein complexes involved in chromatin remodeling (Mi-2β, NURF & NURD), transcriptional repression (Prc2, Sin3) and chromatin assembly (CAF1). Both RbAp46 and RbAp48 are specifically found in the catalytic cores of histone deacetylase (HDAC) complexes that promote transcriptional repression of target genes, including those repressed by the retinoblastoma tumor suppressor protein.

RbAp46 binds to and enhances the activity of the type B histone acetyltransferase HAT1, an enzyme that acetylates histone H4 specifically at its Lys5 and Lys12 residues prior to their incorporation into nucleosomes during replication.

RbAp48 is an evolutionarily conserved subunit of the chromatin assembly factor-1 (CAF-1) complex, where it associates with two other subunits, known as p150 and p60 in human cells. It is also reported that E2F-1 and RbAp48 are physically associated in the presence of Rb and histone deacetylase, suggesting that RbAp48 could be involved in transcriptional repression of E2F-responsive genes.

for RbAp46/48 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RbAp46/48 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RbAp46/48 antibody (pAb).

for RbAp46/48 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RbAp46/48 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

RbAp46/48 antibody (pAb)

Data Thumbnails

RbAp46/48 antibody (pAb) tested by ChIP.

ChIP of RbAp46/48 pAb.
(Click image to enlarge and see details.)

RbAp46/48 antibody (pAb) tested by Western blot.

Western blot of RbAp46/48 pAb.
(Click image to enlarge and see details.)