MBD3 antibody (mAb)

RRID: AB_2615024
Clone: 106B691
Catalog No: 39216 Format: 100 µg ¥70,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This MBD3 antibody was raised against a synthetic peptide corresponding to human MBD3.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 

MBD3 mAb tested by Western blot.
The analysis was performed using HeLa whole-cell extract and MBD3 mAb at 2 µg/ml (lane 1) and a 1 µg/ml (lane 2) dilutions.

Background

MBD3 (methyl-CpG binding domain protein 3) is a subunit of the NuRD, a multi-subunit complex containing nucleosome remodeling and histone deacetylase activities. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD3 regulates transcription by forming a Mi-2/NuRD complex with nucleosome remodeling and histone deacetylase (HDAC) activities in mammalian cells. MBD3 has been shown to interact not with 5-methylcytidine methylated DNA but with DNA containing 5-hydroxymethylcytosine (5-hmC) methylation. MDB3 co-localizes with TET1 and is required for normal levels of 5-hmC in embryonic stem cells.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for MBD3 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting