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NFκB p65 antibody (pAb)

Aliases: RELA, p65, NFKB3
商品コード: 39283 Format: 200 µl ¥68,400 Buy Now
商品コード: 39284 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:65 kDa


Western Blot Validated

for NFkB p65 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

for NFkB p65 antibody (pAb)Immunogen

This NFkB p65 antibody was raised against a peptide immunogen corresponding to N-terminal p65.

for NFkB p65 antibody (pAb)Buffer

Immunoaffinity purified antibody formulated in 30% glycerol containing 0.035% sodium azide.

for NFkB p65 antibody (pAb)References

NFκB p65 antibody (pAb) tested by Western blot.

NFκB p65 pAb tested by Western blot.
NFκB p65 detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and NFκB p65 pAb at a 1:2,000 dilution.

for NFkB p65 antibody (pAb)Background

NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

for NFkB p65 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for NFκB p65 antibody.

for NFkB p65 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p65 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

NFκB p65 antibody (pAb)

Data Thumbnails

NFκB p65 antibody (pAb) tested by Western blot.

Western blot of NFκB p65 pAb.
(Click image to enlarge and see details.)