HOXA9 antibody (pAb)

RRID: AB_2614933
Aliases: HOX1, ABD-B, HOX1G, HOX1.7
Catalog No: 39825 Format: 100 µl ¥86,000 Buy
Catalog No: 39826 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
48 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This HOXA9 antibody was raised against a peptide within the N-terminal region of human HOXA9.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

HOXA9 antibody (pAb) tested by Western blot.
K-562 nuclear extract (20 µg per lane) probed with HOXA9 antibody at a dilution of 1:1,000.

Background

HOXA9 (Homeobox protein A9, HOX1G) is a sequence-specific DNA binding transcription factor with a central role in both hematopoiesis and leukemia. HOXA9 is required for normal hematopoiesis and contributes to myeloid blood cell differentiation. High levels of HOXA9 expression in hematopoietic cells is a characteristic feature of acute myeloid leukemia (AML), and may contribute to the development of this disease. Overexpression of HOXA9 markedly expands hematopoietic stem cells.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for HOXA9 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting