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SALL4 antibody (pAb)
| RRID: AB_2793408 |
| Aliases: Spalt Like Transcription Factor 4, DRRS, HSAL4, ZNF797, dJ1112F19.1 |
| 商品コード: 39957 | Format: 100 µl | ¥89,000 | Buy |
| 商品コード: 39958 | Format: 10 µl | ¥25,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 10 µl per IP
WB*: 1:500 - 1:2,000 dilution
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This SALL4 antibody was raised against a synthetic peptide derived from the N-terminus of human SALL4.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
SALL4 antibody (pAb) tested by Immunoprecipitation.
10 µl of SALL4 antibody was used to immunoprecipitate SALL4 from 500 µg of F9 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SALL4 antibody at a dilution of 1:500.
SALL4 antibody (pAb) tested by Western blot.
Nuclear extract of P19 cells (20 μg) probed with SALL4 antibody (1:500).
Background
SALL4 (Sal-like protein 4) is a zinc-finger transcription factor required for the maintenance of embryonic stem cell pluripotency. SALL4 activates the expression of Oct4 and reduction in the levels of SALL4 eliminates the ability of ES cells to self-renew and causes differentiation. SALL4 mutations are associated with the developmental disorders IVIC syndrome and Duane-radial ray syndrome.
