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Tools to analyze nuclear function,
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MED1 antibody (pAb)

Catalog No: 61065 Format: 100 µl ¥68,400 Buy Now
Catalog No: 61066 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:210 kDa


Western Blot Validated Immunoprecipitation Validated

for MED1 antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for MED1 antibody (pAb)Immunogen

This MED1 antibody was raised against a peptide within the C-terminal region of human MED1.

for MED1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MED1 antibody (pAb)References

MED1 antibody (pAb) tested by immunoprecipitation.

MED1 antibody (pAb) tested by Immunoprecipitation.
10 µl of MED1 antibody was used to immunoprecipitate MED1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MED1 antibody at a dilution of 1:2,000.

MED1 antibody (pAb) tested by Western blot.

MED1 antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of HeLa cells probed with MED1 antibody at a dilution of 1:2,000.

for MED1 antibody (pAb)Background

MED1 (Mediator complex subunit 1, TRIP2, Thyroid receptor-interacting protein 2) is a component of the Mediator complex, a coactivator involved in the regulated transcription of most genes transcribed by RNA polymerase II. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. MED1 is involved in supporting hematopoietic stem and/or progenitor cell function.

for MED1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for MED1 (TRIP2) antibody.

for MED1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MED1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

MED1 antibody (pAb)

Data Thumbnails

MED1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of MED1 pAb.
(Click image to enlarge and see details.)

MED1 antibody (pAb) tested by Western blot.

Western blot of MED1 pAb.
(Click image to enlarge and see details.)