XRCC1 antibody (pAb)

RRID: AB_2793610
Aliases: RCC
商品コード: 61371 Format: 100 µl ¥89,000 Buy
商品コード: 61372 Format: 10 µl ¥25,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
85 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
WB*: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a recombinant protein corresponding to the N-terminal region of human XRCC1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

 
XRCC1 antibody (pAb) tested by immunofluorescence.

XRCC1 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with XRCC1 antibody at a 1:2,000 dilution.

XRCC1 antibody (pAb) tested by Western blot.

XRCC1 antibody (pAb) tested by Western blot.
XRCC1 detection by Western blot. The analysis was performed using 30 µg HeLa nuclear cell extract and XRCC1 at a 1:500 dilution.

Background

XRCC1 (X-ray repair complementing defective repair in Chinese hamster cells 1) corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase (PARP) to participate in the base excision repair (BER) pathway.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for XRCC1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting