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POLR2B antibody (pAb)

Aliases: RPB2, POL2RB, hsRPB2, hRPB140
Catalog No: 61557 Format: 100 µl ¥68,400 Buy Now
Catalog No: 61558 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:135 kDa


Western Blot Validated Immunoprecipitation Validated

for POLR2B antibody (pAb)Application Notes

Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for POLR2B antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human POLR2B.

for POLR2B antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for POLR2B antibody (pAb)References

POLR2B antibody (pAb) tested by immunoprecipitation.

POLR2B antibody (pAb) tested by Immunoprecipitation.
10 µl of POLR2B antibody was used to immunoprecipitate POLR2B from 200 µg of HEK293 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the POLR2B antibody at a dilution of 1:500.

POLR2B antibody (pAb) tested by Western blot.

POLR2B antibody (pAb) tested by Western blot.
Nuclear extract of HEK293 cells (30 µg) probed with POLR2B antibody (pAb) at a dilution of 1:1,500.

for POLR2B antibody (pAb)Background

POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

for POLR2B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for POLR2B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

POLR2B antibody (pAb)

Data Thumbnails

POLR2B antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of POLR2B pAb.
(Click image to enlarge and see details.)

POLR2B antibody (pAb) tested by Western blot.

Western blot of POLR2B pAb.
(Click image to enlarge and see details.)