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AbFlex® Histone H3.3 antibody (rAb)
| RRID: AB_2793796 |
| 商品コード: 91191 | Format: 100 µg | ¥72,000 | Buy |
| 商品コード: 91192 | Format: 10 µg | ¥20,000 | Buy |
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Applications
Immunogen
This antibody was raised against a peptide corresponding to amino acids 21-39 of human Histone H3.3.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
ChIP-Seq of AbFlex Histone H3.3 antibody (rAb)
ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from brain tumor cell cells and 4 µg of AbFlex Histone H3.3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15.1 million sequence tags were mapped to identify Histone H3.3 binding sites. The image shows binding across a region of chromosome 1.
AbFlex® Histone H3.3 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on an SDS-PAGE gel and probed with 2 µg/ml AbFlex Histone H3.3 antibody.
MW: 17 kDa
AbFlex® Histone H3.3 antibody (rAb) tested by Western Blot
125 ng of recombinant H3.1 and recombinant H3.3 were loaded on SDS-PAGe gel and probed with AbFlex® Histone H3.3 antibody at 1 µg/mL.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AbFlex® Histone H3.3 antibody (rAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
