Histone H3K4me1 antibody (mAb)

RRID: AB_2793284
Clone: MABI 0302
Catalog No: 39635 Format: 100 µg ¥72,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 4 µg each
ChIP: 10 µl per ChIP
WB: 1:500 - 1:2,000 dilution

For Histone H3K4me1, we also offer AbFlex® Histone H3K4me1 Recombinant Antibody (rAb). For details, see Catalog No. 91289.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

The Histone H3 monomethyl Lys4 antibody was raised against a peptide containing monomethyl lysine 4 of human histone H3.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Histone H3K4me1 antibody (mAb) tested by ChIP-Seq
ChIP was performed using 10 µg of chromatin from MCF7 cells and 4 µg of antibody. ChIP DNA was sequenced and 19 million sequence tags were mapped to identify Histone H3K4me1 binding sites.

Histone H3 monomethyl Lys4 antibody (mAb) tested by Western blot.
HeLa acid extract probed with Histone H3 monomethyl Lys4 antibody (mAb) (2 µg/ml dilution).

Histone H3 monomethyl Lys4 antibody (mAb) tested by Dot Blot
Dot Blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys4 antibody (mAb) for monomethyl Lys4 histone H3. Methylated peptides corresponding to the immunogen or related peptides were spotted onto PVDF and probed with antibody at 1 µg/ml. Lane 1: unmodified peptide, Lane 2: Monomethyl Lys4, Lanes 3-8: various negative control peptides.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys4 antibody (Clone MABI 0302).

qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me1 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting