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WDR5 antibody (pAb)

Aliases: SWD3, BIG-3
Catalog No: 61485 Format: 100 µl ¥68,400 Buy Now
Catalog No: 61486 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:40 kDa


Western Blot Validated

for WDR5 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for WDR5 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human WDR5.

for WDR5 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for WDR5 antibody (pAb)References

WDR5 antibody (pAb) tested by Western blot.

WDR5 antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of Raji (Lane 1) and HeLa cells (Lane 2) probed with WDR5 antibody (pAb) at a 1:500 dilution.

for WDR5 antibody (pAb)Background

WDR5 (WD repeat-containing protein 5) is a component of the Set1 histone methyltransferase / MLL1/MLL complex that is involved in methylation and dimethylation of 'Lys-4' of Histone H3. H3K4 methylation represents a specific tag for epigenetic transcriptional activation. WDR5 is also a component of the NSL complex and it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. WDR5 is also core component of other methyltransferase-containing complexes including MLL2/3 (also named ASCOM complex) and MLL4/WBP7.

for WDR5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for WDR5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

WDR5 antibody (pAb)

Data Thumbnails

WDR5 antibody (pAb) tested by Western blot.

Western blot of WDR5 pAb.
(Click image to enlarge and see details.)