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PHF8 antibody (pAb)
| RRID: AB_2793316 |
| Aliases: JHDM1F, MRXSSD, ZNF422 |
| 商品コード: 39711 | Format: 100 µl | ¥89,000 | Buy |
| 商品コード: 39712 | Format: 10 µl | ¥25,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This PHF8 antibody was raised against a synthetic peptide derived from human PHF8.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
PHF8 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with PHF8 pAb (1:500 dilution).
Background
PHF8 (PHD finger protein 8) is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 also has an N-terminal PHD finger and demethylates Histone H3 monomethyl Lys9 (H3K9me1) and Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). Defects in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD).
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PHF8 pAb.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
