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Histone H3K9me1 antibody (pAb)
| RRID: AB_2793204 |
| 商品コード: 39249 | Format: 100 µl | ¥69,000 | Buy |
| 商品コード: 39250 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-Seq
WB
This antibody has been published, but has not been not validated for ChIP-Seq by Active Motif. ChIP-validated antibodies are available for this target: Catalog No. 39681.
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 monomethyl Lys9 antibody was raised against a peptide including monomethyl lysine 9 of histone H3.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39887) of this antibody that was purified by Protein A Chromatography is also available.
View our Guide to Histone Modifications and Biological Function.
Histone H3 monomethyl Lys9 pAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 monomethyl Lys9 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 monomethyl Lys9 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 monomethyl Lys9 pAb at a dilution of 1:500.
Histone H3 monomethyl Lys9 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Catalog No. 39249 for monomethyl Lys9 of histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with Catalog No. 39249 at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified lysine 4 peptide. Lane 2: monomethyl lysine 4 peptide. Lane 3: dimethyl lysine 4 peptide. Lane 4: trimethyl lysine 4 peptide. Lane 5: unmodified lysine 9 peptide. Lane 6: monomethyl lysine 9 peptide. Lane 7: dimethyl lysine 9 peptide. Lane 8: trimethyl lysine 9 peptide. Lane 9: unmodified lysine 27 peptide. Lane 10: monomethyl lysine 27 peptide. Lane 11: dimethyl lysine 27 peptide. Lane 12: trimethyl lysine 27 peptide.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me1 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
