Histone H3K9me1 antibody (pAb)

RRID: AB_2793204
Catalog No: 39249 Format: 100 µl ¥70,000 Buy
Catalog No: 39250 Format: 10 µl ¥20,000 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:250 - 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP-Seq


This antibody has not been validated for ChIP-Seq by Active Motif. ChIP-validated antibodies are available for this target: 39681.

Immunogen

This Histone H3 monomethyl Lys9 antibody was raised against a peptide including monomethyl lysine 9 of histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39887) of this antibody that was purified by Protein A Chromatography is also available.

View our Guide to Histone Modifications and Biological Function.

References

 

Histone H3 monomethyl Lys9 pAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 monomethyl Lys9 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H3 monomethyl Lys9 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 monomethyl Lys9 pAb at a dilution of 1:500.

Histone H3 monomethyl Lys9 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Catalog No. 39249 for monomethyl Lys9 of histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with Catalog No. 39249 at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified lysine 4 peptide. Lane 2: monomethyl lysine 4 peptide. Lane 3: dimethyl lysine 4 peptide. Lane 4: trimethyl lysine 4 peptide. Lane 5: unmodified lysine 9 peptide. Lane 6: monomethyl lysine 9 peptide. Lane 7: dimethyl lysine 9 peptide. Lane 8: trimethyl lysine 9 peptide. Lane 9: unmodified lysine 27 peptide. Lane 10: monomethyl lysine 27 peptide. Lane 11: dimethyl lysine 27 peptide. Lane 12: trimethyl lysine 27 peptide.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys9 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting