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Histone H3K36me2 antibody (pAb)
| RRID: AB_2793207 |
| 商品コード: 39255 | Format: 100 µl | ¥92,000 | Buy |
| 商品コード: 39256 | Format: 10 µl | ¥25,000 | Buy |
| 商品コード: 39056 | Format: 50 µl | ¥55,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39891) of this antibody that was purified by Protein A Chromatography is also available.
Histone H3 dimethyl Lys36 antibody tested by ChIP-chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human lymphoma cell line (3 million cells). The ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows H3K36me2 enrichment across the FMNL2 gene.
Histone H3 dimethyl Lys36 antibody tested by immunofluorescence.
Staining of HeLa cells with Histone H3 dimethyl Lys36 antibody (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 dimethyl Lys36 antibody tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 dimethyl Lys36 antibody at a dilution of 1:2,500.
Histone H3K36me2 antibody (pAb) tested by CUT&Tag
CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 6 million reads, and H3K36me2 data is shown for Chromosome 7.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K36me2 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
