Recombinant ALKBH1 protein

Aliases: AlkB Homolog 1, Histone H2A Dioxygenase
Catalog No: 81128 Format: 50 µg ¥76,000 Buy
Catalog No: 81828 Format: 1 mg ¥481,000 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant ALKBH1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.

Background

ALKBH1 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH1 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data this product is shown. 

Protein Details

Full length ALKBH1 protein (accession number NP_006011.2) was expressed in E.coli cells with an N-terminal 6×His-Tag. The molecular weight of ALKBH1 is 47.6 kDa.

 

Recombinant ALKBH1 protein gel
10% SDS-PAGE Coomassie staining
MW: 47.6 kDa
Purity: >60%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.