Recombinant ALKBH7 protein

Catalog No: 81132 Format: 100 µg ¥76,000 Buy
Catalog No: 81832 Format: 1 mg ¥481,000 Buy
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button


Contents

Recombinant ALKBH7 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP.

Background

ALKBH7 (Alkylated DNA Repair Protein AlkB Homolog 7), also called as SPATA11 (Spermatogenesis-Associated Protein 11) is a member of ALKBH protein family. It does not display DNA demethylase activity according to recent research, and may function as protein hydroxylase. It has been proved to catalyze auto-hydroxylation at Leu-110 in vitro. ALKBH7 is required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. It acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length ALKBH7 protein (accession number NP_115682.1) was expressed in E.coli cells with an N-terminal 6Å~His tag. The molecular weights of ALKBH7 is 28.1 kDa.

 

Recombinant ALKBH7 protein gel
12.5% SDS-PAGE Coomassie staining
MW: 37.4 kDa
Purity: >85%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.