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Histone H3S10phS28ph antibody (pAb)
| RRID: AB_2793167 |
| 商品コード: 39147 | Format: 200 µl | ¥69,000 | Buy |
| 商品コード: 39148 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Immunogen
This Histone H3 phospho Ser10,28 antibody was raised against a peptide including phosphorylated serine 28 of human Histone H3.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H3 phospho Ser10,28 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H3 phospho Ser10, 28 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the GAPDH gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3 phospho Ser10,28 antibody tested by immunofluorescence.
HeLa cells stained with Histone H3 phospho Ser10,28 antibody at a 1:1,000 dilution.
Top panel: Histone H3 phospho Ser10,28 antibody.
Middle: DAPI.
Bottom: Merge of both images.
Histone H3 phospho Ser10,28 antibody tested by Western blot.
HeLa acid extract probed with Catalog No. 39147 (1:5,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3S10phS28ph antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
